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ion xpress tm barcode adapters kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher ion xpress tm barcode adapters kit
    Ion Xpress Tm Barcode Adapters Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ion+xpress+tm+barcode+adapters+kit/ion+xpress+barcode+adapters/pmc11781114-161-8-14
    Average 90 stars, based on 1 article reviews
    ion xpress tm barcode adapters kit - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Development of an AmpliSeq TM Panel for Next-Generation Sequencing of a Set of Genetic Predictors of Persisting Pain
    Article Snippet: A total of 10 ng DNA per sample was used for the target enrichment by a multiplex PCR and each DNA pool was amplified with the Ion Ampliseq TM Library Kit in conjunction with the Ion Ampliseq TM “custom Primer Pool”-protocols according to the manufacturer’s procedures (Life Technologies, Darmstadt, Germany). .. After each pool had undergone 18 PCR cycles, the PCR primers were removed with FuPa Reagent and the amplicons were ligated to the sequencing adaptors with short stretches of index sequences (barcodes) that enabled sample multiplexing for subsequent steps (Ion Xpress TM Barcode Adapters Kit; Life Technologies). .. After purification with AMPure XP beads (Beckman Coulter, Krefeld, Germany), the barcoded libraries were quantified with a Qubit ® 2.0 Fluorimeter (Life Technologies, Darmstadt, Germany) and normalized for DNA concentration to a final concentration of 20 pmol/l using the Ion Library Equalizer TM Kit (Life Technologies, Darmstadt, Germany).

    Article Title: Compliance with Nutritional Recommendations and Gut Microbiota Profile in Galician Overweight/Obese and Normal-Weight Individuals
    Article Snippet: For this purpose, the 16S hypervariable regions were amplified with two sets of primers, v2–4–8 and v3–6, 7–9, and the libraries were prepared by using the Ion 16S TM Metagenomics Kit (Life Technologies) and the Ion Xpress TM Plus Fragment Library Kit (Life Technologies). .. Libraries containing equal amounts of PCR products pooled with a barcode were prepared by using the Ion Xpress TM Barcode Adapters Kit (Life Technologies). .. Then, these libraries were quantified by using the Ion Universal Library Quantitation Kit (Life Technologies).

    Sequencing:

    Article Title: Development of an AmpliSeq TM Panel for Next-Generation Sequencing of a Set of Genetic Predictors of Persisting Pain
    Article Snippet: A total of 10 ng DNA per sample was used for the target enrichment by a multiplex PCR and each DNA pool was amplified with the Ion Ampliseq TM Library Kit in conjunction with the Ion Ampliseq TM “custom Primer Pool”-protocols according to the manufacturer’s procedures (Life Technologies, Darmstadt, Germany). .. After each pool had undergone 18 PCR cycles, the PCR primers were removed with FuPa Reagent and the amplicons were ligated to the sequencing adaptors with short stretches of index sequences (barcodes) that enabled sample multiplexing for subsequent steps (Ion Xpress TM Barcode Adapters Kit; Life Technologies). .. After purification with AMPure XP beads (Beckman Coulter, Krefeld, Germany), the barcoded libraries were quantified with a Qubit ® 2.0 Fluorimeter (Life Technologies, Darmstadt, Germany) and normalized for DNA concentration to a final concentration of 20 pmol/l using the Ion Library Equalizer TM Kit (Life Technologies, Darmstadt, Germany).

    Multiplexing:

    Article Title: Development of an AmpliSeq TM Panel for Next-Generation Sequencing of a Set of Genetic Predictors of Persisting Pain
    Article Snippet: A total of 10 ng DNA per sample was used for the target enrichment by a multiplex PCR and each DNA pool was amplified with the Ion Ampliseq TM Library Kit in conjunction with the Ion Ampliseq TM “custom Primer Pool”-protocols according to the manufacturer’s procedures (Life Technologies, Darmstadt, Germany). .. After each pool had undergone 18 PCR cycles, the PCR primers were removed with FuPa Reagent and the amplicons were ligated to the sequencing adaptors with short stretches of index sequences (barcodes) that enabled sample multiplexing for subsequent steps (Ion Xpress TM Barcode Adapters Kit; Life Technologies). .. After purification with AMPure XP beads (Beckman Coulter, Krefeld, Germany), the barcoded libraries were quantified with a Qubit ® 2.0 Fluorimeter (Life Technologies, Darmstadt, Germany) and normalized for DNA concentration to a final concentration of 20 pmol/l using the Ion Library Equalizer TM Kit (Life Technologies, Darmstadt, Germany).



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